Perfusion Process Flowsheets & Simulations
Explore 2 validated, solved Perfusion simulation flowsheets in MaximaLabs — real components: ethanol, water. Open any one directly in your browser.
Solved via: NRTL.
Perfusion bioreactor: continuous mAb culture with cell retention
A continuous mammalian culture run in perfusion — fresh medium is exchanged continuously while an ATF/TFF device retains the cells, and density is set by a deliberate bleed rather than by the medium-exchange rate. That decoupling is the whole point, and it is what an ordinary chemostat cannot represent: at steady state the cell balance pins mu - mu_d to the cell-specific removal rate D_eff = D[phi + (1-phi)(1-R)], not to the dilution rate, so this culture holds roughly ten times the cell density the same feed would support in a chemostat. Lactate accumulates and inhibits growth, cells die at a basal rate (so the reported viability is below 100%), and the secreted antibody follows Luedeking-Piret kinetics — leaving in the cell-free harvest while the bleed carries the cells away. Set retention to 0.0 and this collapses exactly to the plain chemostat the 'bioreactor' example shows.
4 unit ops • NRTL
76 0
View & openContinuous mAb: perfusion bioreactor into TFF concentration
The upstream and downstream halves of a biologics process on one canvas. A perfusion bioreactor produces a cell-free harvest, and the antibody in it is then concentrated by tangential-flow filtration — the step every biologics process ends with. The membrane is a per-species rejection, which is what makes UF/DF a genuine steady-state unit rather than something that has to be pretended into one: the antibody is fully retained while the spent substrate and lactate pass freely into the permeate. The split is not asserted — it follows from the concentration target, and the flux the membrane can actually deliver at the resulting wall concentration is what sets the area. The polarization is the point. Retained protein piles up at the membrane wall far above the bulk, and it is the wall concentration that sets the osmotic back-pressure — which is why ultrafiltration flux plateaus with pressure instead of rising with it, and why a specified-recovery membrane model cannot represent this step at all. Bounded, and inherited from the underlying model: no fouling or time-dependent resistance growth, and the virial coefficients that set a protein's osmotic pressure are caller inputs because they are measured per protein per formulation — no protein databank ships with this.
6 unit ops • NRTL
55 0
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